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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/09/2014 |
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Actualizado : |
18/03/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IBÁÑEZ, F.; REZANO, A.; FREDES, A.; RODRIGUEZ, G.; REGGIO, A.; VILARÓ, F. |
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Afiliación : |
FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANABELA REZANO MATKOVICH, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALEJANDRO DAVID FREDES SIVOPLAS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO ADOLFO RODRIGUEZ TEXEIRA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA MARIA REGGIO BIGANZOLI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Development of a rapid microplate assay for determining pungency in onion breeding program. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Acta Horticulturae (ISHS), 2012, no. 935, p. 61-65. |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2012.935.8 |
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Idioma : |
Inglés |
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Notas : |
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. |
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Contenido : |
ABSTRACT.
Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). |
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Thesagro : |
AJO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02138naa a2200229 a 4500
001 1050611
005 2022-03-18
008 2012 bl uuuu u00u1 u #d
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2012.935.8$2DOI
100 1 $aIBÁÑEZ, F.
245 $aDevelopment of a rapid microplate assay for determining pungency in onion breeding program.$h[electronic resource]
260 $c2012
500 $aXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding.
520 $aABSTRACT. Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95).
650 $aAJO
700 1 $aREZANO, A.
700 1 $aFREDES, A.
700 1 $aRODRIGUEZ, G.
700 1 $aREGGIO, A.
700 1 $aVILARÓ, F.
773 $tActa Horticulturae (ISHS), 2012, no. 935, p. 61-65.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
09/09/2014 |
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Actualizado : |
28/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
LEISOVA, L.; MINARIKOVA, V.; KUCERA, L.; PEREYRA, S. |
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Afiliación : |
SILVIA ANTONIA PEREYRA CORREA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Structure of the Cochliobolus sativus population variability. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Plant Pathology, v. 61, n. 4, p. 709-718, 2012. |
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ISSN : |
1365-3059 |
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DOI : |
10.1111/j.1365-3059.2011.02547.x |
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Idioma : |
Inglés |
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Notas : |
Article history: Issue Online: 17 July 2012 //Version of Record online: 23 October 2011 //Editorial history: Published online 24 October 2011. |
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Contenido : |
The genetic diversity of several local p opulations of the fungal pathogen Cochliobolus sativus, collected over 3 years fromdifferent regions of the Czech Republic, was examined using amplified fragment length polymorphism (AFLP). A high levelof variability was found even among isolates from one lesion. Measures of multilocus linkage disequilibrium suggested thatrecombination has a minor impact on the genetic structure of populations. Coch liobolus sativus forms genetically divergentpopulations ( FST=0Æ33), indi cating a low level of genefl ow between populations. This was supported by a significant corre-lation between genetic diversity and geographical distances up to 80?100 km. The most likely explanation for the geneticvariability is that the fungus forms conidia with highly variable chromo somal rearrangements. The differentiation observedamong local populations implies that genetic drift, including a founder effect, combined with restricted migration generatesthe structure of C. sativus popu lations. |
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Palabras claves : |
AMPLIFIED FRAGMENT LENGTH POLYMORPHISM; BARLEY; COCHLIOBOLUS SATIVUS; DIVERSITY; GENEFLOW; INDEX OF ASSOCIATION; LINKAGE DISEQUILIBRIUM; POPULATION VARIABILITY; WHEAT. |
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Thesagro : |
CEBADA; FITOPATOLOGÍA; TRIGO. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02079naa a2200337 a 4500
001 1050046
005 2018-09-28
008 2012 bl uuuu u00u1 u #d
022 $a1365-3059
024 7 $a10.1111/j.1365-3059.2011.02547.x$2DOI
100 1 $aLEISOVA, L.
245 $aStructure of the Cochliobolus sativus population variability.$h[electronic resource]
260 $c2012
500 $aArticle history: Issue Online: 17 July 2012 //Version of Record online: 23 October 2011 //Editorial history: Published online 24 October 2011.
520 $aThe genetic diversity of several local p opulations of the fungal pathogen Cochliobolus sativus, collected over 3 years fromdifferent regions of the Czech Republic, was examined using amplified fragment length polymorphism (AFLP). A high levelof variability was found even among isolates from one lesion. Measures of multilocus linkage disequilibrium suggested thatrecombination has a minor impact on the genetic structure of populations. Coch liobolus sativus forms genetically divergentpopulations ( FST=0Æ33), indi cating a low level of genefl ow between populations. This was supported by a significant corre-lation between genetic diversity and geographical distances up to 80?100 km. The most likely explanation for the geneticvariability is that the fungus forms conidia with highly variable chromo somal rearrangements. The differentiation observedamong local populations implies that genetic drift, including a founder effect, combined with restricted migration generatesthe structure of C. sativus popu lations.
650 $aCEBADA
650 $aFITOPATOLOGÍA
650 $aTRIGO
653 $aAMPLIFIED FRAGMENT LENGTH POLYMORPHISM
653 $aBARLEY
653 $aCOCHLIOBOLUS SATIVUS
653 $aDIVERSITY
653 $aGENEFLOW
653 $aINDEX OF ASSOCIATION
653 $aLINKAGE DISEQUILIBRIUM
653 $aPOPULATION VARIABILITY
653 $aWHEAT
700 1 $aMINARIKOVA, V.
700 1 $aKUCERA, L.
700 1 $aPEREYRA, S.
773 $tPlant Pathology$gv. 61, n. 4, p. 709-718, 2012.
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